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Antibodies sure to the blocking peptide not bind towards the epitope on the goal protein. By comparing the staining in the blocked antibody vs . the antibody on your own, you can see which staining is certain.
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This guidebook outlines further more achievable optimization of mobile and immunoassay parameters to make sure the very best results are...
This information outlines further more feasible optimization of cellular and immunoassay parameters to guarantee the very best outcomes are...
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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Blocking peptides are peptides that bind especially for the goal antibody and block antibody binding. These peptide generally consists of the epitope recognized by the antibody.
Target Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which contribute to your temporal coordination of each and every mitotic occasion. Cyclin E sorts a posh with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
Overexpression of this gene has been noticed in many tumors, which ends up in chromosome instability, and thus may well contribute to tumorigenesis. This protein was uncovered to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a essential role in advertising and marketing cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two more splice variants were documented but in-depth nucleotide sequence details will not be yet offered.
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